Spekulative Arten

Bei Speculative Evolution haben wir ausgehend von wissenschaftlichen Publikationen über synthetische Biologie, Gentechnik und Robotik überlegt, wie Arten weiterentwickelt werden könnten, um ihre Widerstandsfähigkeit zu erhöhen. Daraufhin haben wir Textanweisungen formuliert, um mit DALL-E KI-generierte Bilder zu erstellen. Jede spekulative Art in der Simulation hat so eine Hintergrundgeschichte, die in realen Szenarien verwurzelt ist.
 
 


Looper Moth
Looper Moths
2007used for production of a recombinant antibody fragment in insect larvae
Laboratory research by O’Connell et al., 2007
2054used for the production of large quantities of recombinant proteins used for human pharmaceuticals

Stammbaum der 66 Arten von insgesamt 92

    • Looper Moth, Art 10-1Samsung G955F, Android 9, Zurich, Switzerland (10-1)
      • Looper Moth, Art 10-1-1Samsung G955F, Android 9, Zurich, Switzerland (10-1-1)
      • Looper Moth, Art 10-1-2Samsung G955F, Android 9, Stuttgart, Germany (10-1-2)
        • Looper Moth, Art 10-1-2-1Samsung G955F, Android 9, Stuttgart, Germany (10-1-2-1)
        • Looper Moth, Art 10-1-2-2Samsung T720, Android 11, Del Viso, Argentina (10-1-2-2)
          • Looper Moth, Art 10-1-2-2-1Samsung T720, Android 11, Jose C. Paz, Argentina (10-1-2-2-1)
    • Looper Moth, Art 10-2Samsung G955F, Android 9, Zurich, Switzerland (10-2)
      • Looper Moth, Art 10-2-1Samsung G955F, Android 9, Zurich, Switzerland (10-2-1)
        • Looper Moth, Art 10-2-1-1Samsung G955F, Android 9, Stuttgart, Germany (10-2-1-1)
          • Looper Moth, Art 10-2-1-1-1Samsung G955F, Android 9, Stuttgart, Germany (10-2-1-1-1)
    • Looper Moth, Art 10-3Samsung G955F, Android 9, Zurich, Switzerland (10-3)
      • Looper Moth, Art 10-3-1Samsung G955F, Android 9, Zurich, Switzerland (10-3-1)
        • Looper Moth, Art 10-3-1-1Samsung A217F, Android 12, , India (10-3-1-1)
      • Looper Moth, Art 10-3-2Samsung G955F, Android 9, Stuttgart, Germany (10-3-2)
        • Looper Moth, Art 10-3-2-1Samsung G998U1, Android 14, Groton, United States (10-3-2-1)
    • Looper Moth, Art 10-4Samsung G955F, Android 9, Zurich, Switzerland (10-4)
      • Looper Moth, Art 10-4-1Xiaomi 5s, Android 6.0.1, Lima, Peru (10-4-1)
        • Looper Moth, Art 10-4-1-1, Android 10, Las Piedras, Uruguay (10-4-1-1)
          • Looper Moth, Art 10-4-1-1-1Samsung G955F, Android 9, Berlin, Germany (10-4-1-1-1)
            • Looper Moth, Art 10-4-1-1-1-1, Android 11, Alor Star, Malaysia (10-4-1-1-1-1)
              • Looper Moth, Art 10-4-1-1-1-1-1Huawei HRY-LX1T, Android 10, Rome, Italy (10-4-1-1-1-1-1)
            • Looper Moth, Art 10-4-1-1-1-2Samsung G955U, Android 9, , China (10-4-1-1-1-2)
              • Looper Moth, Art 10-4-1-1-1-2-1Samsung A536B, Android 14, Tower Hamlets, United Kingdom (10-4-1-1-1-2-1)
                • Looper Moth, Art 10-4-1-1-1-2-1-1Samsung G955U, Android 9, , China (10-4-1-1-1-2-1-1)
                • Looper Moth, Art 10-4-1-1-1-2-1-2Samsung G986U1, Android 13, Monterrey, Mexico (10-4-1-1-1-2-1-2)
      • Looper Moth, Art 10-4-2Samsung T720, Android 11, Del Viso, Argentina (10-4-2)
      • Looper Moth, Art 10-4-3Samsung G950F, Android 9, São Paulo, Brazil (10-4-3)
        • Looper Moth, Art 10-4-3-1Samsung G950F, Android 9, São Paulo, Brazil (10-4-3-1)
      • Looper Moth, Art 10-4-4Samsung G955U, Android 9, , China (10-4-4)
        • Looper Moth, Art 10-4-4-1Samsung G955U, Android 9, , China (10-4-4-1)
    • Looper Moth, Art 10-6Samsung A217F, Android 12, , India (10-6)
      • Looper Moth, Art 10-6-1Samsung A217F, Android 12, , India (10-6-1)
        • Looper Moth, Art 10-6-1-1Samsung G955F, Android 9, Schaffhausen, Switzerland (10-6-1-1)
          • Looper Moth, Art 10-6-1-1-1Samsung G955F, Android 9, Lucerne, Switzerland (10-6-1-1-1)
          • Looper Moth, Art 10-6-1-1-2Samsung G955F, Android 9, Lucerne, Switzerland (10-6-1-1-2)
        • Looper Moth, Art 10-6-1-2Samsung G955U, Android 9, Basel, Switzerland (10-6-1-2)
        • Looper Moth, Art 10-6-1-3Samsung G955F, Android 9, Lucerne, Switzerland (10-6-1-3)
          • Looper Moth, Art 10-6-1-3-1Samsung G955F, Android 9, Lucerne, Switzerland (10-6-1-3-1)
            • Looper Moth, Art 10-6-1-3-1-1Samsung G955F, Android 9, Lucerne, Switzerland (10-6-1-3-1-1)
        • Looper Moth, Art 10-6-1-4Samsung G955F, Android 9, Lucerne, Switzerland (10-6-1-4)
    • Looper Moth, Art 10-7Samsung T720, Android 11, Del Viso, Argentina (10-7)
      • Looper Moth, Art 10-7-1Samsung A505FN, Android 11, Brussels, Belgium (10-7-1)
        • Looper Moth, Art 10-7-1-1Samsung G955U, Android 9, Basel, Switzerland (10-7-1-1)
      • Looper Moth, Art 10-7-2Samsung G955F, Android 9, Lucerne, Switzerland (10-7-2)
        • Looper Moth, Art 10-7-2-1Samsung G955F, Android 9, Lucerne, Switzerland (10-7-2-1)
    • Looper Moth, Art 10-8, Android 13, Bethlehem, United States (10-8)
      • Looper Moth, Art 10-8-1Samsung G955F, Android 9, Limassol, Cyprus (10-8-1)
        • Looper Moth, Art 10-8-1-1Samsung G986U1, Android 13, Monterrey, Mexico (10-8-1-1)
        • Looper Moth, Art 10-8-1-2Samsung G955F, Android 9, Lucerne, Switzerland (10-8-1-2)
          • Looper Moth, Art 10-8-1-2-1Samsung G986U1, Android 13, Monterrey, Mexico (10-8-1-2-1)
        • Looper Moth, Art 10-8-1-3Samsung G955U, Android 9, Xi'an, China (10-8-1-3)
    • Looper Moth, Art 10-9Samsung G950F, Android 9, São Paulo, Brazil (10-9)
      • Looper Moth, Art 10-9-1Samsung G950F, Android 9, São Paulo, Brazil (10-9-1)
        • Looper Moth, Art 10-9-1-1, Android 12, Havana, Cuba (10-9-1-1)
          • Looper Moth, Art 10-9-1-1-1Samsung G986U1, Android 13, Monterrey, Mexico (10-9-1-1-1)
            • Looper Moth, Art 10-9-1-1-1-1Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-1-1-1)
            • Looper Moth, Art 10-9-1-1-1-2Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-1-1-2)
            • Looper Moth, Art 10-9-1-1-1-3Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-1-1-3)
        • Looper Moth, Art 10-9-1-2Samsung G955U, Android 9, , China (10-9-1-2)
          • Looper Moth, Art 10-9-1-2-1Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-2-1)
          • Looper Moth, Art 10-9-1-2-2Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-2-2)
        • Looper Moth, Art 10-9-1-3Samsung G955U, Android 9, , China (10-9-1-3)
          • Looper Moth, Art 10-9-1-3-1Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-3-1)
            • Looper Moth, Art 10-9-1-3-1-1Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-3-1-1)
            • Looper MothSamsung G955F, Android 9, Lucerne, Switzerland (10-9-1-3-1-1)
            • Looper Moth, Art 10-9-1-3-1-3Samsung G955F, Android 9, Lucerne, Switzerland (10-9-1-3-1-3)

Production of a recombinant antibody fragment in whole insect larvae

O'Connell, Kevin P et al., Molecular biotechnology vol. 36,1 (2007): 44-51. doi:10.1007/s12033-007-0014-4
https://pubmed.ncbi.nlm.nih.gov/17827537/

Abstract

Infection of insect cells with baculovirus expression constructs is commonly used to produce recombinant proteins that require post-translational modifications for their activity, such as mammalian proteins. However, technical restraints limit the capacity of insect cell-based culture systems to be scaled up to produce the large amounts of recombinant protein required for human pharmaceuticals. In this study, we designed an automated insect rearing system and whole insect baculovirus expression system (PERLXpress) for the expression and purification of recombinant proteins on a large scale. As a test model, we produced a recombinant mouse anti-botulinum antibody fragment (Fab) in Trichoplusia ni larvae. A recombinant baculovirus co-expressing the Fab heavy and light chains together with N-terminal sequences from the silkworm hormone bombyxin, to direct proteins into the secretory pathway, was constructed. Fifth instar larvae were reared and infected orally with recombinant (pre- occluded) baculovirus using the automated system and harvested approximately after 4 days. The total yield of recombinant Fab was 1.1 g/kg of larvae, resulting in 127 mg of pure Fab in one production run. The Fab was purified to homogeneity using immobilized metal affinity chromatography, gel filtration, and anion exchange chromatography. The identity of the purified protein was verified by Western blots and size-exclusion chromatography. Purified recombinant Fab was used to detect botulinum toxin in ELISA experiments, demonstrating that the heavy and light chains were properly assembled and folded into functional heterodimers. We believe that this is the first demonstration of the expression of a recombinant antibody in whole insect larvae. Our results demonstrate that a baculovirus-whole larvae expression system can be used to express functionally active recombinant Fab fragments. As the PERLXpress system is an automated and linearly scalable technology, it represents an attractive alternative to insect cell culture for the production of large amounts of human pharmaceuticals.